plasmid pegip Search Results


egfp  (Addgene inc)
93
Addgene inc egfp
Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/egfp/product/Addgene inc
Average 93 stars, based on 1 article reviews
egfp - by Bioz Stars, 2026-05
93/100 stars
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tgfp  (Addgene inc)
93
Addgene inc tgfp
a, Schematic of gene targeting <t>by</t> <t>HDR,</t> PITCh, or HITI in the GFP-correction HEK293 line. Blue pentagon, Cas9/gRNA target sequence. Black line within blue pentagon, Cas9 cleavage site. HITI donors: IRESmCherry-0c, IRESmCherry-1c, IRESmCherry-2c or IRESmCherry-MC. HDR-donors: <t>tGFP</t> and IRESmCherry-HDR-0c. A PITCh-donor: IRESmCherry-MH. The green square and triangle, CRISPR/Cas9 target sites to create 8-bp microhomology at both ends of the IRESmCherry cassette. HDR and HITI dual donors: IRESmCherry-HDR-2c. Genome cut-only control donor: IRESmCherry-0c. Donor cut-only control donor: IRESmCherry-MC-scramble. Red pentagon, Scramble-gRNA target sequence. b, Gene targeting efficiency by HDR, PITCh, or HITI (with or without indels at junction sites). Results were obtained from three replicate wells and presented as mean ± s.d. The input data points were shown as black dots. c, Schematic of targeted GFP knock-in by HDR, PITCh, or HITI in cultured primary neurons. d, Experimental scheme for GFP knock-in in cultured primary neurons. e, Representative immunofluorescence images of neurons transfected with Tubb3-GFP HITI constructs: BPNLS-Cas9, Tubb3gRNA-mCherry, and Tubb3-MC plasmids. Inset, higher magnification image. Scale bar, 100 μm. f, Targeted GFP knock-in efficiencies indicated by percentage of GFP+ cells among transfected cells (mCherry+) or all cells (DAPI+) in EdU+ or EdU− neurons. n, technical replicates. Results were presented as mean ± s.d. For source data, see Supplementary Table 1.
Tgfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tgfp/product/Addgene inc
Average 93 stars, based on 1 article reviews
tgfp - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier

Image Search Results


a, Schematic of gene targeting by HDR, PITCh, or HITI in the GFP-correction HEK293 line. Blue pentagon, Cas9/gRNA target sequence. Black line within blue pentagon, Cas9 cleavage site. HITI donors: IRESmCherry-0c, IRESmCherry-1c, IRESmCherry-2c or IRESmCherry-MC. HDR-donors: tGFP and IRESmCherry-HDR-0c. A PITCh-donor: IRESmCherry-MH. The green square and triangle, CRISPR/Cas9 target sites to create 8-bp microhomology at both ends of the IRESmCherry cassette. HDR and HITI dual donors: IRESmCherry-HDR-2c. Genome cut-only control donor: IRESmCherry-0c. Donor cut-only control donor: IRESmCherry-MC-scramble. Red pentagon, Scramble-gRNA target sequence. b, Gene targeting efficiency by HDR, PITCh, or HITI (with or without indels at junction sites). Results were obtained from three replicate wells and presented as mean ± s.d. The input data points were shown as black dots. c, Schematic of targeted GFP knock-in by HDR, PITCh, or HITI in cultured primary neurons. d, Experimental scheme for GFP knock-in in cultured primary neurons. e, Representative immunofluorescence images of neurons transfected with Tubb3-GFP HITI constructs: BPNLS-Cas9, Tubb3gRNA-mCherry, and Tubb3-MC plasmids. Inset, higher magnification image. Scale bar, 100 μm. f, Targeted GFP knock-in efficiencies indicated by percentage of GFP+ cells among transfected cells (mCherry+) or all cells (DAPI+) in EdU+ or EdU− neurons. n, technical replicates. Results were presented as mean ± s.d. For source data, see Supplementary Table 1.

Journal: Nature

Article Title: In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration

doi: 10.1038/nature20565

Figure Lengend Snippet: a, Schematic of gene targeting by HDR, PITCh, or HITI in the GFP-correction HEK293 line. Blue pentagon, Cas9/gRNA target sequence. Black line within blue pentagon, Cas9 cleavage site. HITI donors: IRESmCherry-0c, IRESmCherry-1c, IRESmCherry-2c or IRESmCherry-MC. HDR-donors: tGFP and IRESmCherry-HDR-0c. A PITCh-donor: IRESmCherry-MH. The green square and triangle, CRISPR/Cas9 target sites to create 8-bp microhomology at both ends of the IRESmCherry cassette. HDR and HITI dual donors: IRESmCherry-HDR-2c. Genome cut-only control donor: IRESmCherry-0c. Donor cut-only control donor: IRESmCherry-MC-scramble. Red pentagon, Scramble-gRNA target sequence. b, Gene targeting efficiency by HDR, PITCh, or HITI (with or without indels at junction sites). Results were obtained from three replicate wells and presented as mean ± s.d. The input data points were shown as black dots. c, Schematic of targeted GFP knock-in by HDR, PITCh, or HITI in cultured primary neurons. d, Experimental scheme for GFP knock-in in cultured primary neurons. e, Representative immunofluorescence images of neurons transfected with Tubb3-GFP HITI constructs: BPNLS-Cas9, Tubb3gRNA-mCherry, and Tubb3-MC plasmids. Inset, higher magnification image. Scale bar, 100 μm. f, Targeted GFP knock-in efficiencies indicated by percentage of GFP+ cells among transfected cells (mCherry+) or all cells (DAPI+) in EdU+ or EdU− neurons. n, technical replicates. Results were presented as mean ± s.d. For source data, see Supplementary Table 1.

Article Snippet: We have confirmed that the Scramble-gRNA can cut its target site in the donor vector ( ). pMDLg/pRRE, pRSV-Rev and pMD2.G (Addgene 12251, 12253 and 12259) were used for packaging lentiviruses. pEGIP*35 and tGFP (Addgene 26776 and 26864) were used for examining HDR and HITI efficiencies.

Techniques: Sequencing, CRISPR, Control, Knock-In, Cell Culture, Immunofluorescence, Transfection, Construct